The purpose of this research was to formulate and systemically evaluate in-vitro performances of Floating Mucoadhesive Ramipril microspheres for its potential use in the treatment of hypertension, myocardial infarction. Ramipril Mucoadhesive microspheres were prepared by an emulsion-solvent evaporation technique containing HPMC K4M and chitosan as mucoadhesive polymer and ethyl cellulose and calcium carbonate as buoyancy increasing floating agent. Preformulation studies were carried out before formulation design. Total nine formulations were prepared. Microspheres were discrete, spherical, free-flowing and The prepared microspheres were subjected to evaluation for a good percentage of drug entrapment efficiency. particle size, in vitro buoyancy, in vitro mucoadhesion and in vitro drug release characteristics. An in-vitro wash off test showed that Ramipril mucoadhesive microspheres adhered more strongly to the gastric mucous layer and could be retained in the gastrointestinal tract for an extended period of time. In-vitro dissolution test was carried out by using phosphate pH 2. All the formulations showed good dissolution profiles. Among all the formulation F5 showed good dissolution profile with 78.25% of drug release in 12 hours. In-vitro release kinetic data of Ramipril microspheres showed that the drug release mechanism was diffusion controlled as the plots of peppas model was linear. The results showed a sustained anti-hypertensive effect over a longer period of time in case of mucoadhesive microspheres, compared to the powder. In conclusion, the prolonged gastrointestinal residence time and slow release of Ramipril resulting from the Mucoadhesive microspheres could contribute to the provision of a sustained anti-hypertensive effect.
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